alpha-N-acetylgalactosaminidase from the limpet, Patella vulgata.
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چکیده
منابع مشابه
cw-IV-Acetylgalactosaminidase from the Limpet, Patek vulgata*
a-N-Acetylgalaetosaminidase, devoid of P-N-acetylhexosaminidase, has been purified 860-fold from the limpet, Patella uulgata. The final preparation gives one protein band on disc gel electrophoresis, sodium dodecyl sulfate-disc gel electrophoresis, and disc gel isoelectrofocusing. By Sephadex G-ZOO filtration, the molecular weight of this enzyme is 200,000 at pH 4.2 and is 45,000 at pH 7.0. The...
متن کاملPurification and properties of alpha-D-mannosidase from the limpet, Patella vulgata.
1. alpha-Mannosidase from the limpet, Patella vulgata, was purified nearly 150-fold, with 40% recovery. beta-N-Acetylglucosaminidase was removed from the preparation by treatment with ethanol. The final product was virtually free from beta-galactosidase. 2. Limpet alpha-mannosidase was assayed at pH3.5 and at this pH it was necessary to add Zn(2+) for full activity. At pH5, the enzyme had the s...
متن کاملcw - IV - Acetylgalactosaminidase from the Limpet ,
a-N-Acetylgalaetosaminidase, devoid of P-N-acetylhexosaminidase, has been purified 860-fold from the limpet, Patella uulgata. The final preparation gives one protein band on disc gel electrophoresis, sodium dodecyl sulfate-disc gel electrophoresis, and disc gel isoelectrofocusing. By Sephadex G-ZOO filtration, the molecular weight of this enzyme is 200,000 at pH 4.2 and is 45,000 at pH 7.0. The...
متن کاملAssessment of heavy metal contamination of seawater and marine limpet, Patella vulgata L., from Northwest Spain.
In the present work, both seawater and limpets (Patella vulgata L.) were collected from different sampling points in the Vigo estuary area (Galicia, NW Spain) and their heavy metal concentrations (Zn, Cd, Pb, Cu) were determined by means of a voltammetric technique. The results showed a direct relationship between the heavy metal levels in seawater and the content in biological samples, especia...
متن کاملThe Golgi Apparatus in Neurons and Epithelial Cells of the Common Limpet Patella Vulgata
1. In view of widely diverse views held about the identity and structure of the Golgi apparatus in neurons of Mollusca, particularly gastropods, a study has been made on neurons of the common limpet, Patella vulgata, both by light and electron microscopy. A report is given also of observations made on epithelial cells of Patella by electron microscopy. 2. As revealed by Kolatchev's method, the ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)63330-2